| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 1878251 | Applied Radiation and Isotopes | 2008 | 7 Pages |
Abstract
The monoclonal antibody TU-20 and its scFv fragment were radiolabeled with 125I in order to develop new imaging agents against the specific neuronal marker III β-tubulin. The reaction via chloramine-T using thiosulfate as a stopping reductant was determined as the most convenient way for radioiodination. The preserved immunological properties of radioiodinated species were estimated by ELISA, electrophoresis, and immunohistochemistry with autoradiography. Biodistribution studies revealed a different behavior of radioiodinated TU-20 and its scFv.
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Authors
Helena Å vecová, Veronika Kleinová, Daniel Seifert, Hana Chaloupková, Jörg Bäurle, Karel Kranda, Vlastimil Král, Miroslav FiÅ¡er,