| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 1880305 | Physica Medica | 2015 | 8 Pages |
•NTHSP significantly improved the GSH-Px activity and increased GSH and ceruloplasmin contents in vivo.•NTHSP obviously protected the DNA against the damages induced by radiation.•NTHSP effectively blocked the mitochondrial apoptotic pathway induced by radiation.
In this study, the radioprotective effect of neutral polysaccharides from Hohenbuehelia serotina (NTHSP) against the damages induced by 60Co-γ radiation was investigated. The results showed that NTHSP could significantly improve the activity of glutathione peroxidase (GSH-Px) and increase the contents of glutathione (GSH) and ceruloplasmin in plasma after treated with 6 Gy-radiation compared with the radiation controls (p < 0.05). Furthermore, administration with NTHSP could effectively increase the quantity of marrow DNA (p < 0.05) and reduce the rates of chromosome aberration and micronuclei (p < 0.01) in bone marrows of mice. In addition, NTHSP could markedly inhibit the expressions of Bax protein and promote the expressions of Bcl-2 protein, accordingly inhibit the releases of cytochrome c and expressions of activated Caspase-3, and therefore block the mitochondrial apoptotic pathway of splenocytes in mice induced by 60Co-γ radiation. These results suggested that NTHSP might be a natural radioprotective agent against the injuries induced by radiation.
