| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 188990 | Electrochimica Acta | 2012 | 5 Pages |
The poly (diallyldimethylammonium chloride) (PDDA) functionalized graphene nanosheets (PDDA-G) were prepared and gold nano-particles (AuNPs) were assembled on the surface of PDDA-G film through electrostatic interaction to construct the biocompatible interface. The resulting AuNPs/PDDA-G nanocomposite film exhibited enhanced capability for hemoglobin (Hb) immobilization and realized its direct electrochemistry. The surface concentration (Γ*) of electroactive Hb on the surface of GCE was calculated to be 3.85 × 10−9 mol/cm2. Moreover, the AuNPs/PDDA-G based sensor showed prominent electrocatalytic activity for the detection of H2O2 with a wide linear range from 6 μM to 1010 μM and a low detection limit of 0.39 μM at 3σ. Meanwhile, the apparent Michaelis–Menten constant (Km) was 0.51 mM. This biosensor exhibited high sensitivity and fast responses, which may provide a novel way for fabrication of other graphene based sensors and broaden the application of graphene in biosensing.
Graphical abstractFigure optionsDownload full-size imageDownload as PowerPoint slideHighlights► AuNPs/PPDA-G multi-material has been fabricated which has high biocompatibility. ► AuNPs/PPDA-G multi-nanomaterials exhibited enhanced capability for hemoglobin immobilization. ► The larger surface concentration (Γ*) of electroactive Hb was 3.85 × 10−9 mol/cm2. ► The biosensor showed prominent electrocatalytic activity for H2O2. ► The lower apparent Michaelis–Menten constant (Km) was 0.51 mM, shown hemoglobin retaining its high bioactivity for H2O2 oxidation.
