Electrochemical determination of anticancer drug fulvestrant at dsDNA modified pencil graphite electrode

The interaction of fulvestrant with double-stranded DNA (dsDNA) was studied by means of differential pulse voltammetry (DPV) at dsDNA modified pencil graphite electrode (PGE). The decrease in intensity of the guanine oxidation signals was used as an indicator for the interaction mechanism and sensitive determination of fulvestrant in pH 4.80 acetate buffer (30% ethanol). A linear dependence of the guanine oxidation signals was observed within the range of 1.00–20.00 μg/mL fulvestrant, with a detection limit of 0.41 μg/mL. The determination method was validated according to the standard validation procedure. The proposed method was applied to fulvestrant pharmaceutical dosage form.

► Fulvestrant is new type of estrogen receptor antagonist in the treatment of postmenopausal women with advanced breast cancer. ► Electrochemical approach can provide new insights into rational drug design and will lead to further understanding of the mechanism of interaction between anticancer drugs and DNA. ► There is an interaction between Fulvestrant and dsDNA by voltammetry based on guanine signal. ► The decreasing of guanine signal with different concentration fulvestrants, could be explained by the damage on the oxidizable group of guanine because of the adsorption of Fulvestrant.