| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 1908654 | Free Radical Biology and Medicine | 2012 | 7 Pages |
Abstract
⺠Evidence of
- NO-mediated protein radical formation in HRas is presented. ⺠Immuno-spin trapping (IST) and tandem mass spectrometry indicate a Cys118 Ras protein radical. ⺠IST may be used to detect thiyl radical formation in the Ras oncoprotein. ⺠The IST approach described can be used for other nonmetalloproteins.
- NO-mediated protein radical formation in HRas is presented. ⺠Immuno-spin trapping (IST) and tandem mass spectrometry indicate a Cys118 Ras protein radical. ⺠IST may be used to detect thiyl radical formation in the Ras oncoprotein. ⺠The IST approach described can be used for other nonmetalloproteins.
Keywords
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Authors
Michael F. Davis, Li Zhou, Marilyn Ehrenshaft, Kalina Ranguelova, Harsha P. Gunawardena, Xian Chen, Marcelo G. Bonini, Ronald P. Mason, Sharon L. Campbell,