| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 1908936 | Free Radical Biology and Medicine | 2012 | 9 Pages |
Abstract
⺠We studied mitochondrial morphology in endothelial cells exposed to simulated I/RP. ⺠Reperfusion was simulated as flow of oxygenated media (shear stress) over the cells. ⺠Mitochondria underwent extensive fission following endothelial exposure to I/RP. ⺠Mitochondrial fission was dependent on NO, ROS (including mtROS) and Drp1. ⺠We conclude that mechanical forces can play a role on mitochondrial dynamics.
Keywords
MFNmitochondrial division inhibitor-1mtPTPM199OxaloacetateOAATMRMNG-nitro-l-arginine methyl estermtROSC-PTIOPBSMdivi-1Drp1ΔΨmNACHUVECN-acetyl-l-cysteineeNOS2-(4-carboxyphenyl)-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxideopa1l-NAMEO2•−ONOO−β-mercaptoethanolβ-Meoptic atrophy 1mitochondrial permeability transition porehuman umbilical vein ECischemia/reperfusionMitochondrial fissionShear stressFree radicalselectron transport chainEndothelial cellendothelial nitric oxide synthaseSuperoxideMitochondrial superoxidemedium 199tetramethylrhodamine methyl esterPhosphate-buffered salineMitofusinNitric oxidehypoxia/reoxygenationETcMitochondrial inner membrane potentialdynamin-related protein 1Peroxynitritemitochondrial reactive oxygen species
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Authors
Randy J. Giedt, Changjun Yang, Jay L. Zweier, Anastasios Matzavinos, B. Rita Alevriadou,