Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1909662 | Free Radical Biology and Medicine | 2010 | 7 Pages |
Abstract
Nitric oxide (â
NO) is a ubiquitous signaling molecule that participates in the neuromolecular phenomena associated with memory formation. In the hippocampus, neuronal â
NO production is coupled to the activation of the NMDA-type of glutamate receptor. Although â
NO-mediated signaling has been associated with soluble guanylate cyclase activation, cytochrome oxidase is also a target for this gaseous free radical, for which â
NO competes with O2. Here we show, for the first time in a model preserving tissue cytoarchitecture (rat hippocampal slices) and at a physiological O2 concentration, that endogenous NMDA-evoked â
NO production inhibits tissue O2 consumption for submicromolar concentrations. The simultaneous real-time recordings reveal a direct correlation between the profiles of â
NO and O2 in the CA1 subregion of the hippocampal slice. These results, obtained in a system close to in vivo models, strongly support the current paradigm for O2 and â
NO interplay in the regulation of cellular respiration.
Keywords
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Authors
Ana Ledo, Rui Barbosa, Enrique Cadenas, João Laranjinha,