| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 1913374 | Journal of the Neurological Sciences | 2014 | 4 Pages |
•MS is considered as a chronic inflammatory disease of the central nervous system.•The both nuclear and mitochondrial genes are involved in MS development.•The mtDNA of complex I gene variations are involved in the progression of MS.•Relationship between mitochondrial complex I gene variations and MS.
Background and purposeMultiple sclerosis (MS) is an autoimmune-mediated inflammatory and debilitating disease of the central nervous system. Several investigations have suggested that the mitochondrial DNA encoded subunits of complex I gene variations are involved in the progression of MS. In this study, we investigated the possible association between mitochondrial complex I gene variations and MS in a Filipino population.Material and methodsA total of 300 individuals were included in the present study, two-hundred patients with MS clinical symptoms, and one-hundred healthy subjects without MS clinical features. We amplified target genes of mtDNA using polymerase chain reaction technique (PCR), and sequenced these to evaluate mitochondrial complex I gene variations.ResultsWe found nine variations (Nt 4216 T > C, Nt 5153 A > G, Nt 10142 C > T, Nt 11353 T > C, Nt 11935 T > C, Nt 12062 C > T, Nt 13042 G > A, Nt 13708 G > A and Nt 14179 G > A) in mtDNA-encoded complex I subunit genes. Our results showed that the prevalence of ND1, ND2, ND3, ND4 and ND5 gene variations was significantly higher in patients than in healthy controls (P < 0.0001). Whereas, the frequency of Nt 14179 G > A variation in ND6 gene was significantly higher in the control group compared with the patients (P < 0.0001).ConclusionTaken together our data supports a strongly positive association between mitochondrial complex I gene variations and MS pathogenesis in a Filipino population.
