Article ID Journal Published Year Pages File Type
1919848 Mechanisms of Ageing and Development 2008 9 Pages PDF
Abstract

A number of model systems have been employed to investigate age-associated changes in immune function. The purpose of the current study was to characterize senescent T cells and to investigate the inflamm-aging phenomenon both in vitro and in vivo using the old horse as a model. We examined whether decreased T cell proliferation induced by Con A is caused by increased apoptosis. We also utilized intracellular CFSE to analyze changes within each round of cell proliferation, in particular cytokine production. Intracellular staining with flow cytometry, RT-PCR, and ELISA were used to measure pro-inflammatory cytokines both in vitro and in vivo. While lymphocytes from old horses exhibit decreased proliferation, this is not the result of increased apoptosis. Instead, a larger percentage of the T cells remain in the parent generation and produce significant amounts of IFNγ. Likewise, old horses have increased frequency of CD8−IFNγ+ T cells and TNFα producing cells. We also show that old horses have elevated levels of IL-1β, IL-15, IL-18 and TNFα gene expression in peripheral blood and significant levels of TNFα protein in serum, all characteristics of inflamm-aging.

Related Topics
Life Sciences Biochemistry, Genetics and Molecular Biology Ageing
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