Oxidized lipids activate autophagy in a JNK-dependent manner by stimulating the endoplasmic reticulum stress response

Article ID	Journal	Published Year	Pages	File Type
1923233	Redox Biology	2013	9 Pages	PDF

Abstract

âº The lipid peroxidation product 4-hydroxynonenal (HNE) modifies endoplasmic reticulum (ER) proteins in vascular smooth muscle cells. âº HNE activates autophagy. âº PKR-like ER kinase (PERK) and the stress kinase JNK are activated by HNE. âº Pharmacological inhibition of JNK or ER stress prevents autophagy in vascular smooth muscle cells.

Keywords

PVDF NP-40 ECL PDI HRP UPR IRE DTT eIF2α DNPH LC3 HEPES RASMC 4-hydroxynonenal GRP FBS Jnk SDS DMEM ATF6 HNE 2,4-dinitrophenylhydrazine 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid 4-hydroxy-trans-2-nonenal c-Jun N-terminal kinase DMSO PKR-like ER kinase Autophagy enhanced chemiluminescence Oxidative stress dithiothreitol Dimethylsulfoxide sodium dodecyl sulfate fetal bovine serum Rat aortic smooth muscle cells Smooth muscle cells endoplasmic reticulum activating transcription factor 6 Unfolded protein response Horseradish peroxidase glucose regulated protein protein disulfide isomerase microtubule-associated protein 1 light chain 3 PERK Polyvinylidene fluoride