Article ID Journal Published Year Pages File Type
193498 Electrochimica Acta 2008 6 Pages PDF
Abstract

A combination of a selective enzymatic oxidation with an unselective electrochemical reduction step was applied for deracemization, stereoinversion and asymmetric synthesis of l-leucine (starting from racemic leucine, d-leucine or 4-methyl-2-oxovaleric acid) in a batch reactor. d-Amino acid oxidase (d-AAO) from Trigonopsis variabilis was used as enzyme. Reaction conditions for the electrochemical and enzymatic reactions were investigated separately and finally combined to an electroenzymatic synthesis, yielding 3.5 mmol L−1 d−1 of l-leucine (ee 91%).

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