The electrochemical response of microperoxidase in non-aqueous solvents

Microperoxidase-11 (MP-11) was immobilised at gold electrodes and characterised using UV–vis and resonance Raman spectroscopy. On immersion in a range of non-aqueous solvents, no significant spectroscopic changes were observed. The redox potential of MP-11 was determined in 16 solvents and ranged from −384 to −228 mV. The redox potential was independent of the dielectric constant and hydrophobicity of the solvents examined.