Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
19738 | Food Bioscience | 2013 | 10 Pages |
The capability of anthocyanins extract of Lannea microcarpa fruits to scavenge reactive oxygen species and stall aflatoxin B1-mediated oxidative rout on cellular proteins, lipids and DNA was investigated. Reactive oxygen species (ROS) scavenging potentials of the anthocyanins extract of Lannea microcarpa fruits (0.2–1.0 mg/mL) was investigated using 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical, superoxide ion, hydrogen peroxide, hydroxyl radical and ferric ion reducing system. Anthocyanins extract at 1.0 mg/mL scavenged DPPH, superoxide ion, hydrogen peroxide, and hydroxyl radical by 91%, 88%, 90% and 83%, respectively, it also reduced ferric ion significantly. The specific activities of reactive oxygen species detoxifying enzymes (superoxide dismutase, catalase, glutathione peroxidase, glutathione reductase and glucose 6-phosphate dehydrogenase) were significantly (P<0.05) induced by Lannea microcarpa fruits anthocyanins. These inductions significantly (P<0.05) attenuated the aflatoxin B1-mediated decrease in reactive oxygen species detoxifying enzymes and compared favorably with Vitamin C. Aflatoxin B1-mediated elevation in the concentrations of conjugated dienes, lipid hydroperoxides, malondialdehyde, protein carbonyl and fragmented DNA were significantly (P<0.05) lowered by Lannea microcarpa fruits anthocyanins. Overall, the results of this study show that Lannea microcarpa fruits anthocyanins possesses antioxidant properties and halted aflatoxin B1-mediated oxidative rout on cellular proteins, lipids and DNA.