Down-regulated expression of NPM1 in IMS-M2 cell line by (−)-epigallocatechin-3-gallate

ABSTRACTObjectiveTo investigate the inhibited effect of epigallocatechin-3-gallate (EGCG) on the expression of NPM1 in IMS-M2 cells harboring the NPM1 mutations.MethodsCell proliferation assay was performed to test the effects of EGCG on cell growth of IMS-M2 cells harboring the NPM1 mutations. Western blot analysis were performed to test the protein expression of NPM1, AKT, those associated with apoptosis.ResultsEGCG can down-regulate the expression of NPM1 in IMS-M2 cells harboring the NPM1 mutations. Moreover, EGCG also suppressed the cell proliferation and induced apoptosis in IMS-M2 cells.ConclusionsThe results suggested that EGCG could be considered as a reagent for treatment of AML patients with NPM1 mutations.