| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 2034216 | Biologicals | 2012 | 4 Pages |
Abstract
A fast ELISA was developed and qualified for analysis of polio D-antigen. The original 20 h-protocol was optimized by minimizing the total incubation time to 1 h, and by replacing the signal reagent 3,3′,5,5′-tetramethylbenzidine by a chemiluminogenic signal reagent with a theoretical low intrinsic background and high dynamic range.
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Authors
R. ten Have, Y.E. Thomassen, M.R.J. Hamzink, W.A.M. Bakker, O.E.M. Nijst, G. Kersten, G. Zomer,