Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
2034281 | Biologicals | 2010 | 9 Pages |
Abstract
Effects of 18 commercial lots of fetal calf serum (FCS) after γ-irradiation and their non-irradiated counterparts were comparatively analyzed on CHO-K1 and MDBK MDL1 cells for genotoxicity [sister chromatid exchange (SCE), micronuclei (MNi), and single cell gel electrophoresis (SCGE)], cytotoxicity [cell-cycle progression (CCP), proliferative replication index (PRI), mitotic index (MI), growth promotion (GP), and plating efficiency (PE)], and microbiological properties (mycoplasma and bovine viral diarrhea virus contamination). SCE and SCGE were the most informative end-points for genotoxicity since significant differences were found in 44.4% (P < 0.05-0.001, Student's t-test) and 61.1% (P < 0.05-0.001, Ï2 test) samples, respectively. MI was the cytotoxicity assay revealing the greatest variation, showing differences in 66.7% (P < 0.05-0.001, Ï2 test) samples. Thus, these three end-points for screening bioproducts such as FCS were found most suitable for detecting potential geno-cytotoxicants in biological samples; their simultaneous use could be strongly recommended.
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Authors
Juan Pablo Pilili, Norma V. González, Gabriela Molinari, Miguel A. Reigosa, Sonia Soloneski, Marcelo L. Larramendy,