Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
2034531 | Biologicals | 2008 | 9 Pages |
Abstract
Quantitative anti-F1 and anti-V IgG enzyme-linked immunosorbent assays (ELISAs) were developed to measure the serological response of female Swiss Webster mice after vaccination with the recombinant fusion protein, rF1-V, which is being developed as a plague vaccine. Several fundamental parameters of the ELISA were evaluated: specificity, precision, accuracy, and stability. Experimental results suggested that a potency assay based upon the serological response of female Swiss Webster mice, as measured by quantitative anti-F1 IgG and anti-V IgG ELISAs, might be used to evaluate the rF1-V fusion protein vaccine.
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Authors
S.F. Little, W.M. Webster, H. Wilhelm, B. Powell, J. Enama, J.J. Adamovicz,