Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
2034556 | Biologicals | 2008 | 6 Pages |
Abstract
Seoul virus glycoprotein Gn is a major structural protein and candidate antigen of hantavirus that induces a highly immunogenic response for hantavirus vaccine. In this study, a replication-competent recombinant canine adenovirus type-2 expressing Gn was constructed by the in vitro ligation method. The Gn expression cassette, including the human cytomegalovirus (hCMV) promoter/enhancer and the SV40 early mRNA polyadenylation signal, was cloned into the SspI site of the E3 region which is not essential for proliferation of CAV-2. Expression of Gn was confirmed by reverse transcription-polymerase chain reaction (RT-PCR) and Western blotting.
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Authors
Ziguo Yuan, Xiuxiang Zhang, Shoufeng Zhang, Ye Liu, Shengyan Gao, Fei Zhang, Huijuan Xu, Xiaohu Wang, Rongliang Hu,