Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
2034560 | Biologicals | 2008 | 5 Pages |
Abstract
Some extraction techniques (SDS/chloroform and toluene) should never be used: the measurements are neither precise nor accurate. Bugbuster extraction combined with UV280 quantification gives the best results, followed by the combinations Sonication-UV280 and EasyLyse-UV280. However, if one does not want to use the quantification method UV280, one can opt to use Bugbuster, EasyLyse or sonication extraction combined with any quantification method with exception of the EasyLyse-BCA_P and Sonication-BCA_P combinations.
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Authors
Marjan De Mey, Gaspard J. Lequeux, Jo Maertens, Cassandra I. De Muynck, Wim K. Soetaert, Erick J. Vandamme,