RETRACTED: Cytohesins Are Cytoplasmic ErbB Receptor Activators

This article has been retracted: please see Elsevier Policy on Article Withdrawal (http://www.elsevier.com/locate/withdrawalpolicy).This article has been retracted at the request of the authors.In Bill et al., we identified the cytohesin ARNO as an ErbB receptor activator that enhances receptor autophosphorylation in H460 and SkBr3 cells. Cell-free reconstitution with purified proteins showed that ARNO enhances EGF receptor autophosphorylation by direct interaction with the intracellular domain of the receptor (EGFR-ICD). We were recently notified by Cell that the laboratory of Dr. Oreste Segatto was unable to replicate these effects in HeLa cells or under cell-free reconstituted conditions. Although we reproducibly observe 1.6-fold ARNO-dependent stimulation of cellular EGFR autophosphorylation and partial colocalization of ARNO with EGFR in plasma membrane sheets of HeLa cells, we cannot reproduce the data showing direct stimulation of EGFR-ICD autophosphorylation by ARNO-Sec7 in a cell-free reconstitution system (shown in Figures 5D and S5D). Thus, the conclusion that ARNO enhances receptor activation by direct interaction with EGFR-ICD is no longer supported by experimental evidence. We maintain the view that cellular EGFR autophosphorylation is enhanced by ARNO, that ARNO and EGFR colocalize at the plasma membrane, and that ARNO binds to the EGFR-ICD. Nevertheless, our inability to reproduce the data in Figures 5D and S5D compromises our confidence in the proposed mechanism of this regulation. Therefore, we wish to retract the paper. We apologize for any inconvenience that may have resulted from its publication. Franziska Thorwith could not be reached about this retraction.