Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
2036206 | Cell | 2010 | 12 Pages |
SummaryOpposing roles of Aurora kinases and protein phosphatase 1 (PP1) during mitosis have long been suggested. Here, we demonstrate that Aurora kinases A and B phosphorylate a conserved residue on the kinetochore motor CENP-E. PP1 binds CENP-E via a motif overlapping this phosphorylation site and binding is disrupted by Aurora phosphorylation. Phosphorylation of CENP-E by the Auroras is enriched at spindle poles, disrupting binding of PP1 and reducing CENP-E's affinity for individual microtubules. This phosphorylation is required for CENP-E-mediated towing of initially polar chromosomes toward the cell center. Kinetochores on such chromosomes cannot make subsequent stable attachment to spindle microtubules when dephosphorylation of CENP-E or rebinding of PP1 to CENP-E is blocked. Thus, an Aurora/PP1 phosphorylation switch modulates CENP-E motor activity as an essential feature of chromosome congression from poles and localized PP1 delivery by CENP-E to the outer kinetochore is necessary for stable microtubule capture by those chromosomes.
Graphical AbstractFigure optionsDownload full-size imageDownload high-quality image (208 K)Download as PowerPoint slideHighlights► PP1 binds CENP-E and binding is disrupted by Aurora phosphorylation ► Aurora kinases phosphorylate the kinetochore motor CENP-E at the spindle poles ► Phosphorylation of CENP-E is required for the congression of polar chromosomes ► Binding of PP1 to CENP-E is required for the stable attachment of these chromosomes