Microbiota-Inducible Innate Immune Siderophore Binding Protein Lipocalin 2 Is Critical for Intestinal Homeostasis

Article ID	Journal	Published Year	Pages	File Type
2040694	CMGH Cellular and Molecular Gastroenterology and Hepatology	2016	23 Pages	PDF

Abstract

Lcn2 is a bacterially induced, MyD88-dependent protein that plays an important role in gut homeostasis and a pivotal role on challenge. Hence, therapeutic manipulation of Lcn2 levels may provide a strategy to help manage diseases driven by alteration of the gut microbiota.

Keywords

NGAL IBD RBC DSS MYD88 APP TLR FITC CD45 FBS mAb DMEM rRNA PBS TNF FACS RAG1 LPS LCN2 cDNA MPO Complementary DNA Dulbecco's modified Eagle Medium Ribosomal RNA Monoclonal antibody Colitis Innate immunity interleukin ELISA Enzyme-linked immunosorbent assay Toll-like receptor Germ free cluster of differentiation 45 fetal bovine serum intestinal epithelial cell Dextran sulfate sodium Siderocalin tumor necrosis factor fluorescein isothiocyanate fluorescence-activated cell sorter neutrophil gelatinase-associated lipocalin lipopolysaccharide Lipocalin 2 Phosphate-buffered saline Germ-free mice myeloperoxidase Gut microbiota Neutrophils wild type polymerase chain reaction PCR myeloid differentiation primary response gene 88 Acute phase protein Recombination-activating gene 1 IEC COH red blood cell interleukin-10 receptor