Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
2054857 | International Journal of Medical Microbiology | 2011 | 6 Pages |
Abstract
We evaluated newly developed probes for rapid identification of Burkholderia (B.) pseudomallei and B. mallei and differentiation from B. thailandensis by fluorescence in situ hybridization (FISH). FISH correctly identified 100% of the tested B. pseudomallei (11), B. mallei (11), and B. thailandensis (1) strains, excluded 100% of all tested negative controls (61), and allowed demonstration of B. pseudomallei infection in a paraffin-embedded spleen tissue sample of an experimentally infected mouse.
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Authors
Ralf M. Hagen, Hagen Frickmann, Mandy Elschner, Falk Melzer, Heinrich Neubauer, Yves P. Gauthier, Paul Racz, Sven Poppert,