Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
2058725 | MethodsX | 2014 | 6 Pages |
Abstract
Comet-FISH technique is an extension of commonly used comet assay. Its purpose is to determine whether primary DNA damage which comet assay detects occurred within a sequence of interest that is visualized by hybridization of fluorescent probe. Presence of the signal in comet tail indicates impaired structural integrity of sequence. Our modifications to the original comet-FISH technique described by Rapp et al. (2000) [1] include:•increase in probe binding specificity,•increased rate of successful hybridization,•simultaneous temperature denaturation of both, slide and probe.
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Authors
Marin Mladinic, Davor Zeljezic,