Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
2065276 | Toxicon | 2009 | 5 Pages |
Abstract
HF3 and bothropasin are P-III hemorrhagic snake venom metalloproteinases (SVMPs) of Bothrops jararaca. The DC protein is composed of the disintegrin-like/cysteine-rich domains derived from the autolysis of P-III SVMPs. Here we describe simplified procedures for the isolation of HF3, bothropasin, the DC protein, and BJ-PI, a novel P-I SVMP. The isolated proteins were identified by mass spectrometry. BJ-PI is a potent caseinolytic enzyme devoid of hemorrhagic activity. HF3, bothropasin and BJ-PI show distinct fibrinogenolytic activities.
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Authors
Ana K. Oliveira, Adriana F. Paes Leme, Marina T. Assakura, Milene C. Menezes, André Zelanis, Alexandre K. Tashima, Mônica Lopes-Ferreira, Carla Lima, Antonio C.M. Camargo, Jay W. Fox, Solange M.T. Serrano,