| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 2065357 | Toxicon | 2010 | 10 Pages |
The aim of the present study was to investigate the cardiovascular activity of Scorpaena plumieri venom in both in vivo and in vitro models. In anesthetized rats, doses of the venom (14–216 μg protein/kg) induced a transient increase in the mean arterial pressure. However at higher dose (338 μg protein/kg) this effect was followed by a sudden hypotension and the animal evolved to death. The heart rate was temporarily increased and followed by bradycardia using doses ≥108 μg/kg. In isolated rat hearts the crude venom (5–80 μg protein) produced dose-dependent positive ventricular chronotropic, inotropic, lusitropic and coronary vasoconstriction responses. Partial purification of an active fraction (CF, cardiovascular fraction) which reproduced the cardiovascular effects induced by crude venom on isolated hearts was achieved by conventional gel filtration chromatography. Adrenergic blockades, prazosin and propranolol, significantly attenuated these responses. The coronary vasoconstriction response to CF was also attenuated by chemical endothelium denudation. In conclusion, the data showed that S. plumieri fish venom induces disorders in the cardiovascular system. It also suggests that α1 and β-adrenergic receptors, and the vascular endothelium, are involved at least partially, in these cardiac effects.
