Toxicity of venoms from vipers of Pelias group to crickets Gryllus assimilis and its relation to snake entomophagy

The existing data indicate that snake venom is most toxic towards the natural vertebrate preys. Several species of snake include arthropods in their food. However, there is no available data on the toxicity of venom from entomophagous snakes towards their prey. We have studied the toxicity of venom from vipers of Pelias group towards crickets Gryllus assimilis. The Pelias group includes several closely related viper species inhabiting mainly the South European part of Russia, and they differ in their feeding preferences. Snakes from the Vipera renardi, Vipera lotievi, Vipera kaznakovi, and Vipera orlovi species feed on wide range of animals including insects, whereas snakes from Vipera berus and Vipera nikolskii species do not include insects in their diet. We have found that the venom from vipers that include insects in their diet possesses greater toxicity towards crickets. The greatest toxicity was observed for the venom from V. lotievi, which displays a preference for insects in its diet. Therefore, based on our data, we suggest that the viper entomophagy is not a result of behavior plasticity, but is probably determined at a genetic level.