Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
2067047 | Cell Biology International | 2009 | 6 Pages |
Abstract
Carbonic anhydrase IX (CAIX) is a membrane-associated carbonic anhydrase (CA) that is overexpressed in a variety of tumor types and associated with increased metastasis, giving a poor prognosis. Transcriptional regulation of transmembrane protein CAIX is complex. We describe further characterization of the 1.2 kb hCA9 promoter, and the effect of TGF-β on the transcriptional activity and expression of hCAIX in Hep3B cells. Transcriptional activity of different promoter regions of hCA9 promoter showed the presence of negative regulatory region between â300 bp and â500 bp of hCAIX promoter. The â116/+38 region was enough for basal transcriptional activity in Hep3B cells. TGF-β upregulates all promoter regions of hCA9 with the highest beig for -466/+38 that has a negatively regulated region. The transcriptional activation of hCA9 promoter by TGF-β is consistent with hCAIX mRNA levels revealed by RT-PCR and hCAIX protein expression levels by flow cytometry in Hep3B cells.
Related Topics
Life Sciences
Biochemistry, Genetics and Molecular Biology
Biophysics
Authors
Hatice Yıldırım, Feray Köçkar,