Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
2067282 | Cell Biology International | 2008 | 9 Pages |
Abstract
We have previously shown that human bone cells express bone morphogenetic protein receptor-IB (BMPR-IB). However, little is known about the precise role of this receptor in the response of osteoblastic genes to the BMP in these cells. To determine BMPR-IB-dependent osteoblastic gene expression, the present study examined the effects of BMPR-IB knockdown on BMP-induced osteoblast-associated genes. BMPR-IB mRNA and protein were markedly suppressed by transfection of cells with BMPR-IB siRNA. Using three different bone cell samples, BMP-2 stimulation of alkaline phosphatase (ALP), osteocalcin (OC), distal-less homeobox-5 (Dlx5) and core binding factor alpha-1 (Cbfa1) was found to be specifically and significantly reduced in the BMPR-IB siRNA-transfected cultures compared with that of control cultures. Our study has provided evidence that BMPR-IB-dependent signaling plays a crucial role in BMP-2 up-regulation of the ALP, OC, Dlx5 and Cbfa1 genes in bone cells, suggesting a pivotal role of this receptor in BMP-2-induced osteoblast differentiation in vitro. These findings thus suggest the possibility that BMPR-IB could be a therapeutic target for enhancing bone regeneration in vivo.
Keywords
TGF-βBMP-2RANKLType-I collagenMsx2Smurf1BSPCbfa1distal-less homeobox 5BMPRDlx5OPGRNA interferenceSmall interfering RNART–PCRsiRNAALPAlkaline phosphataseOsteoprotegerinOsteopontinOsteocalcinImmunofluorescenceTransforming growth factor βBone cellsbone sialoproteinBMPreverse transcription–polymerase chain reactionWestern blottingbone morphogenetic proteinsCol Ibone morphogenetic protein receptorreceptor activator of nuclear factor-κB ligand
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Authors
W. Singhatanadgit, V. Salih, I. Olsen,