Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
2067311 | Cell Biology International | 2008 | 7 Pages |
Abstract
We have used a uniform design to explore the most effective directed differentiation medium (MEDDM) for differentiating mouse bone marrow mesenchymal stem cells (mMSCs) into hepatocytes. Our methods involved arranging eight differentiation medium groups following uniform design. Flow cytometry was used to evaluate the percentage of ALB+ and CK18+ cells in each group. Factors and their concentrations in the MEDDMs were then identified. The MEDDMs were evaluated by their ability to differentiate mMSCs into hepatocytes by RNA and protein expressions and synthesis functions. FGF at 35Â ng/ml and OSM at 30Â ng/ml in the medium yielded the highest percentage of ALB+ and CK18+ cells. During directed differentiation using MEDDMs, ALB, CK18, TTR, AFP mRNAs were expressed. ALB and CK18 proteins were detected in the cells. The differentiated cells produced albumin and urea in a time dependent manner. Uniform design was adequate for choosing the MEDDM of mMSCs. MEDDM containing 35Â ng/ml FGF and 30Â ng/ml OSM was effective in differentiating mMSCs into hepatocytes.
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Authors
Xiao-Lei Shi, Liang Mao, Bi-Yun Xu, Ting Xie, Zhang-Hua Zhu, Jun-Hao Chen, Lei Li, Yi-Tao Ding,