Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
2067757 | Cell Biology International | 2007 | 5 Pages |
Abstract
In this study we focused on the early fate of injected human adult hematopoietic stem cells (HSCs). HSCs were followed immunohistochemically 1-19Â h after injection into murine blastocysts. We found that they only rarely attached and integrated into the blastocysts. The high rate of loss of injected cells after prolonged in vitro culture of the chimeras can be explained by apoptosis. Our findings are consistent with previous studies reporting a low rate of integration of adult cells injected to produce chimeric embryos, but this is the first demonstration that the low efficiency of adult stem cell injections into blastocysts is influenced by apoptosis.
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Authors
S. Koestenbauer, P. Vanderzwalmen, A. Hammer, L. Schoonjans, S. Danloy, H. Zech, G. Dohr, N.H. Zech,