Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
2067880 | Cell Biology International | 2006 | 8 Pages |
Abstract
For morphological analysis, microscopic inspection was carried out during cultivation. Cell-associated and extracellular amount of Taxol were detected and measured using HPLC methodology. At stage I, overall Taxol amount of biomass growth medium was 13.75Â mg/l (i.e., 5.6-fold higher than that of untreated B5 control). At stage II, treated cells with methyl jasmonate (10Â mg/l), salicylic acid (100Â mg/l) and fungal elicitor (25Â mg/l) produced the highest amount of Taxol (39.5Â mg/l), which is 16-fold higher than that of untreated B5 control (2.45Â mg/l). Microscopic analyses of Taxus cells in suspension cultures showed various positional auto-fluorescence showing direct correlation with Taxol production. Our studies revealed that intervallic supplementation of B5 medium with combination of biomass growth factors at stage I and mixture of elicitors at stage II could significantly increase Taxol production. Thus, we suggest that the exploitation of this methodology may improve the production of Taxol since demands for Taxol pharmaceuticals are increasingly growing and resource paucities have limited its direct harvesting from Taxus trees.
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Authors
A. Yari Khosroushahi, M. Valizadeh, A. Ghasempour, M. Khosrowshahli, H. Naghdibadi, M.R. Dadpour, Y. Omidi,