Effect of myeloperoxidase and anoxia/reoxygenation on mitochondrial respiratory function of cultured primary equine skeletal myoblasts

Horses are particularly sensitive to excessive inflammatory reaction where myeloperoxidase, a marker of inflammation, may contribute to mitochondrial dysfunctions. This study investigated the interaction between myeloperoxidase and cultured primary equine skeletal myoblasts, particularly its effect on mitochondrial respiration combined or not with anoxia followed by reoxygenation (AR). We showed that active myeloperoxidase entered into the cells, interacted with mitochondria and decreased routine and maximal respirations. When combined with AR, myeloperoxidase caused a further decrease of these respiratory parameters while the leak increased. Our results indicate that myeloperoxidase amplifies the mitochondrial damages initiated by AR phenomenon and alters the mitochondrial function.

► Myeloperoxidase enters into cultured primary equine skeletal myoblasts. ► Myeloperoxidase decreases routine and maximal respirations of the cultured cells. ► Myeloperoxidase amplifies the mitochondrial damages initiated by anoxia/reoxygenation. ► Myeloperoxidase with anoxia/reoxygenation increase the non-efficient respiration. ► Myeloperoxidase with anoxia/reoxygenation alter the mitochondrial function.