Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
2069421 | Mitochondrion | 2007 | 8 Pages |
Abstract
The mitochondrion and the apicoplast of the malaria parasite, Plasmodium spp. is microscopically observed in a close proximity to each other. In this study, we tested the suitability of two different separation techniques – Percoll density gradient centrifugation and fluorescence-activated organelle sorting – for improving the purity of mitochondria isolated from the crude organelle preparation of Plasmodium falciparum. To our surprise, the apicoplast was inseparable from the plasmodial mitochondrion by each method. This implies these two plasmodial organelles are bound each other. This is the first experimental evidence of a physical binding between the two organelles in Plasmodium.
Related Topics
Life Sciences
Biochemistry, Genetics and Molecular Biology
Biophysics
Authors
Tamaki Kobayashi, Shigeharu Sato, Shinzaburo Takamiya, Kanako Komaki-Yasuda, Kazuhiko Yano, Ayami Hirata, Izumi Onitsuka, Masayuki Hata, Fumika Mi-ichi, Takeshi Tanaka, Toshiharu Hase, Atsushi Miyajima, Shin-ichiro Kawazu, Yoh-ichi Watanabe,