| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 2069593 | Mitochondrion | 2011 | 7 Pages |
Abstract
To investigate the set of mtDNA molecules contained in small biological structures, powerful techniques for separation are required. We tested flow cytometry (FCM1), laser capture microdissection (LCM2) and a method using optical tweezers (OT3) in combination with a 1μ-Ibidi-Slide with regard to their ability to deposit single mitochondrial particles. The success of separation was determined by real-time quantitative PCR (qPCR4) and sequencing analysis.OT revealed the highest potential for the separation and deposition of single mitochondrial particles. The study presents a novel setup for effective separation of single mitochondrial particles, which is crucial for the analysis of single mitochondria.
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Authors
René Pflugradt, Ulrike Schmidt, Benjamin Landenberger, Timo Sänger, Sabine Lutz-Bonengel,