Article ID Journal Published Year Pages File Type
2071095 Advanced Drug Delivery Reviews 2012 12 Pages PDF
Abstract

In the last two decades, the single particle and single molecule approach became more and more popular to investigate the activity and the mechano-chemical properties of biological molecules. The inherent limit of these assays was that the molecules of interest were observed in vitro, out of their natural environment, the cell. Several recent works have shown the possibility to overcome this limit, to extend this approach to living cells and to observe the details of many cellular processes at the molecular level.In this review we discuss the use of semiconductor quantum dots to perform single particle and single molecule tracking in the cell. We refer to other articles for the technical aspects of this method. Here, after an introduction on the advantages provided by these nanoparticles, we restrict ourselves to some examples, mainly related to intracellular transport and molecular motor activity. These will illustrate the important role played by semiconductor quantum dots as fluorescent nano-reporters in in cell single molecule approach in modern biology and biophysics.

Graphical abstractFigure optionsDownload full-size imageDownload high-quality image (868 K)Download as PowerPoint slide

Related Topics
Life Sciences Biochemistry, Genetics and Molecular Biology Biotechnology
Authors
, ,