Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
2078881 | Chinese Journal of Biotechnology | 2008 | 7 Pages |
Abstract
Bacillus pumilus X-6-9, isolated from soil and subsequently identified, produced xylooligosaccharides with long chains from xylan and accumulated them in the culture. By improving the culture conditions and mutating the bacterium, a 3.2-fold increase in the production of the xylooligosaccharides was established when compared with the original culture conditions of B. pumilus X-6-19. The addition of D-glucose to the culture of the mutant strain U-3 of B. pumilus X-6-9 repressed the synthesis of β-xylosidase but not xylanase. For these reasons, it was revealed that strain U-3 was a good organism for the production and accumulation of xylooligosaccharides with long chains from xylan by a microbial culture. Xylanase produced by strain U-3 was purified to homogeneity and characterized. The hydrolyzates generated by the purified xylanase contained xylobiose, xylotriose, xylotetraose, and xylopentaose but not xylose.
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Authors
Qingzhu Yuan, Tsuyoshi Adachi, Shinji Takenaka, Shuichiro Murakami, Machiko Tanaka, Kenji Aoki,