Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
2079066 | Chinese Journal of Biotechnology | 2006 | 5 Pages |
Abstract
This study focused on cloning human interleukin-26 (hIL-26) and expressing it in Escherichia coli (E. coli) efficiently. Two pairs of primers were synthesized in accordance with the hIL-26 gene reported by GenBank. The hIL-26 gene was cloned by nest PCR following the first round RT-PCR from human peripheral blood monocytes total RNA, and then the PCR product was cloned into pMD18-T vector. Colony PCR, restriction analysis, and sequence analysis showed that the cloned gene was the same as the reported hIL-26. The recombinant was cut with BamH I and EcoR I to obtain the hIL-26 fragment. The fragment was then inserted into pBV220 that was cut with the same enzymes. The recombinant expression vector was induced to express hIL-26 at 42 °C. SDS-PAGE analysis showed that the recombinant protein accounted for up to 20% of the whole protein of E. coli, and the protein was confirmed by Western blotting. Purity of the protein was found to be above 90% after purification with a molecular sieve. After renaturalization with glutathione buffer, its promoting effect on the production of IFN-γ in PBMC was detected by RT-PCR. A recombinant bacterial strain for expressing hIL-26 with biological activity was constructed successfully.
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Authors
LIU Yi-Qing, CHEN Zi-Jiang, ZHANG Xue, WANG Lai-Cheng, JIAO Yu-Lian, ZHANG Jie, MA Chun-Yan, CUI Bin, GAO Xin-Pu, LIU Zheng-Min, WU Kan, ZHAO Yue-Ran,