| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 2080066 | Drug Discovery Today | 2014 | 8 Pages |
•Hydrogen/deuterium exchange mass spectrometry (HDX-MS) technology for assessment of higher order structure of protein therapeutics.•Methodology: global HDX-MS and peptide HDX-MS.•Applications: epitope mapping and comparability study.
The higher order structure of protein therapeutics can be interrogated with hydrogen/deuterium exchange mass spectrometry (HDX-MS). HDX-MS is now a widely used tool in the structural characterization of protein therapeutics. In this review, HDX-MS based workflows designed for protein therapeutic discovery and development processes are presented, focusing on the specific applications of epitope mapping for protein/drug interactions and biopharmaceutical comparability studies. Future trends in the application of HDX-MS in protein therapeutics characterization are also described.
