B-cell epitope engineering: A matter of recognizing protein features and motives

Using in vivo and in vitro studies B-cell epitopes have been identified on a number of proteins. These epitopes were used to develop predictive methods. After comparison of existing and emerging technologies, this review concludes that antigenicity is not described by physicochemical and structural characteristics of a protein alone. Molecular characteristics of the antigenic amino acids are required. How the structural context affects the selection of these amino acids by the antibody is unknown.

Section editors:Marco van de Weert and Eva Horn Moeller – Department of Pharmaceutics and Analytical Chemistry, Faculty of Pharmaceutical Sciences, University of Copenhagen, Copenhagen, Denmark