Potential and limitations of genetic manipulation in animals

Over the last decade, sequencing and characterisation of the mouse genome has been accompanied by unparalleled advances in functional genomics. In the context of drug action, we analyse the strengths and limitations of classical mutagenesis and gene targeting techniques, as well as alternative approaches such as chemical mutagenesis, gene trap, recombineering, transposon-mediated mutagenesis, chromosomal engineering, viral transgenesis and RNA interference. This review also focuses on the emerging importance of genetic manipulation in other species and related logistical issues of experimental work using mutants.

Section editors:Bart Ellenbroek – Radboud University, Nijmegen, The NetherlandsTwan Ederveen – N.V. Organon, Oss, The Netherlands