Transcutaneous DNA immunization following waxing-based hair depilation elicits both humoral and cellular immune responses

Previously, we showed that transcutaneous (TC) DNA immunization by applying plasmid DNA onto a mouse skin area wherein the hair follicles were induced into growth stage by plucking the hair using warm waxing induced strong and functional antigen-specific antibody responses. In the present study, using plasmids that encode β-galactosidase gene or ovalbumin (OVA) gene, we showed that this mode of TC DNA immunization not only induced specific antibody responses, but also induced antigen-specific cytotoxic T lymphocyte responses. In fact, TC DNA immunization using a plasmid that encodes OVA gene prevented the growth of OVA-expressing B16-OVA tumor cells in the immunized mice. Moreover, we provided additional evidence supporting that hair follicles are essential for this mode of TC DNA immunization.

Graphical abstractDNA immunization by applying plasmid DNA topically onto a mouse skin area wherein the hair had been plucked by warm waxing induced specific antibody and cellular immune responses.Figure optionsDownload full-size imageDownload high-quality image (88 K)Download as PowerPoint slide