Article ID Journal Published Year Pages File Type
2088215 Journal of Immunological Methods 2014 7 Pages PDF
Abstract

Magnetic activated cell sorting (MACS) is a commonly used cell separation technique. Miltenyi Biotec has developed an efficient MACS protocol for isolation of CD8α+ T cells from adult mice spleen. However, due to a low percentage of CD8α+ T cells in spleen of postnatal mice, MACS isolation of CD8α+ T cells results in a low purity. Our study illustrates how this problem can be solved by performing a CD45R+ B cell depletion prior to a positive selection of CD8α+ T cells. This protocol can be used when low frequencies of CD8α+ T cells are present in a tissue also populated with CD45R+ B cells, and a high purity is required for downstream applications.

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