Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
2088215 | Journal of Immunological Methods | 2014 | 7 Pages |
Abstract
Magnetic activated cell sorting (MACS) is a commonly used cell separation technique. Miltenyi Biotec has developed an efficient MACS protocol for isolation of CD8α+ T cells from adult mice spleen. However, due to a low percentage of CD8α+ T cells in spleen of postnatal mice, MACS isolation of CD8α+ T cells results in a low purity. Our study illustrates how this problem can be solved by performing a CD45R+ B cell depletion prior to a positive selection of CD8α+ T cells. This protocol can be used when low frequencies of CD8α+ T cells are present in a tissue also populated with CD45R+ B cells, and a high purity is required for downstream applications.
Keywords
Related Topics
Life Sciences
Biochemistry, Genetics and Molecular Biology
Biotechnology
Authors
Anastasia Hübner, Katja Derkow, Anja U. Bräuer,