Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
2088519 | Journal of Immunological Methods | 2012 | 6 Pages |
Abstract
Antigenic epitopes recognized by FoxP3+ regulatory T cells (Treg) are poorly defined, largely due to a lack of assays for determining Treg specificity. We have developed a novel approach for detecting human Treg specific to peptide antigen, utilizing down-regulation of surface CD3 as a read-out of antigen recognition. Culture conditions and re-stimulation time have been optimized, allowing the detection of even very rare Treg, such as those specific to tumor antigens.
Related Topics
Life Sciences
Biochemistry, Genetics and Molecular Biology
Biotechnology
Authors
Lisa M. Ebert, Sarah E. MacRaild, Ian D. Davis, Jonathan Cebon, Weisan Chen,