Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
2088574 | Journal of Immunological Methods | 2011 | 5 Pages |
Abstract
Discordances between minimal residual disease estimates obtained by different methods are a problem in childhood acute lymphoblastic leukemia. We aimed to optimize methods allowing the biological exploration of such discrepancies, i.e. the combination of flow-sorting of small immunophenotypically defined cell populations with subsequent analyses of leukemia-associated cytogenetic and molecular marker. The approaches described here optimize the use of the same tube of unfixed, antibody-stained BM cells for flow-sorting of small cell populations and subsequent exploratory FISH and PCR-based analyses.
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Authors
Nina Friesgaard Ă˜bro, Hans Ole Madsen, Lars Peter Ryder, Mette Klarskov Andersen, Kjeld Schmiegelow, Hanne Vibeke Marquart,