Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
2088622 | Journal of Immunological Methods | 2010 | 9 Pages |
Abstract
Natural CD4+CD25+Foxp3+ regulatory T cells (Tregs) control the activation of the immune system and therefore have become a major area of research in immunology. The generation of monoclonal antibodies against human Tregs offers the possibility to discover novel Treg-specific or Treg-associated surface markers and to identify targets for a therapeutic modulation of Tregs. Here we present a methodology optimized to efficiently induce and select mAb against human Tregs by repeated immunization of mice with Tregs from a single donor and a differential two-step flow cytometry-based hybridoma screening procedure.
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Authors
Christian Becker, Heinz Hoschützky, Wolfgang Rist, Martin Lenter, Franz-Joseph Schneider, Helmut Jonuleit,