Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
2089034 | Journal of Immunological Methods | 2007 | 8 Pages |
Abstract
Detection of cytotoxic activity by pathogen-specific T cells of unknown antigenic specificity is difficult due to the limitations of using infected cells, instead of peptide-pulsed cells, as targets. We report here that the recently described CD107 mobilization assay readily allowed for the ex vivo detection of cytotoxic T lymphocytes (CTL) with a novel MHC-Ib restriction that specifically recognized Listeria monocytogenes-infected macrophages. The CD107 mobilization assay is likely to be a useful tool for detection of CD8+ T cells that recognize a wide variety of intracellular pathogens.
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Authors
Denise S. McElroy, Adina M. Badstibner, Sarah E.F. D'Orazio,