| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 2089052 | Journal of Immunological Methods | 2007 | 5 Pages |
Abstract
A new simple multi-well plate-based assay to determine monocyte differentiation-inducing activity was developed. It is based on the increase of macrophage adherence after the induction of monocyte differentiation. The assay was conducted using a human monocytic leukemia cell line, THP-1, treated with either 12-O-tetradecanoylphorbol 13-acetate or cytokines, such as tumor necrosis factor-α and interferon-γ. This method is sensitive and easy to perform, especially in case of analyzing a large number of samples.
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Authors
Noboru Uchide, Hiroo Toyoda,