Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
2089092 | Journal of Immunological Methods | 2007 | 9 Pages |
Abstract
A new method for isolation and characterization of peptides presented in the context of the nonclassical human leukocytes antigen (HLA) class I molecule HLA-E was developed. A combination of different chromatographic steps coupled with electrospray mass spectrometry allowed us to detect the presence of small amounts of a naturally processed human Cytomegalovirus (HCMV)-derived peptide isolated from the HEK-293T/HLA-E+/UL40+ transfected cells of from HELA cell line. The peptide sequence was confirmed by tandem mass spectrometry (MS/MS). This approach provides a versatile and sensitive method for direct identification of MHC class I-binding peptides that might be derive from different pathogen or tumor-associated proteins.
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Authors
E. Millo, G. Pietra, A. Armirotti, P. Vacca, M.C. Mingari, L. Moretta, G. Damonte,