Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
2089214 | Journal of Immunological Methods | 2007 | 7 Pages |
Abstract
We have expanded the application of antibody phage display to a new type of antigen: ribonucleoprotein (RNP) complexes. We describe a simple and efficient method for screening antibodies specific for large intact RNPs and individual components. We also describe a fast and easy method to overcome the abundance of amber stop codons in the positive phage clones. The resulting antibodies have been used in ELISA and Western blot analysis.
Keywords
GMPPCPphosphate buffered saline with tween-20IPTGDTTsignal recognition particle receptorscFvTMBTBSHRPRNPSRPPBSTAlkaline phosphataseenzyme linked immunosorbent assayTris buffered salineELISARoom temperaturedithiothreitolsignal recognition particleribonucleoproteinAntibody phage displaySingle chain variable fragmentMacromolecular complexeshorse radish peroxidase
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Authors
Si Wu, Ailong Ke, Jennifer A. Doudna,